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(AV. Luc) or the construct with a minimal promoter (AV. SV (P) Luc) alone gave only minimal expression at 3 days after infection (Fig. 3a). However, co-infection with AV. SupEnh produced 16-fold and 35-fold increases in luciferase expression from the AV. Luc and AV. SV (P) Luc vectors, respectively. These data support our initial hypothesis that cis activation of a minimal SV40 promoter can occur by coinfection with a second AAV vector containing enhancer elements. Unexpectedly, however, there was high-level transactivation of the AV. Luc construct, which contains no heterologous promoter. These findings support earlier studies indicating that ITRs contain a cryptic promoter1. The specificity of this induction was further demonstrated by the lack of transactivation after co-administration of the negative control AV. AmpOri virus with AV. Luc or AV. SV (P) Luc vectors.

To measure the efficiency of the dual-vector …