作者
Gareth R Willis, Monica Reis, Ali Hashemi Gheinani, Angeles Fernandez-Gonzalez, Elizabeth S Taglauer, Vincent Yeung, Xianlan Liu, Maria Ericsson, Eric Haas, S Alex Mitsialis, Stella Kourembanas
发表日期
2021/12/15
期刊
American Journal of Respiratory and Critical Care Medicine
卷号
204
期号
12
页码范围
1418-1432
出版商
American Thoracic Society
简介
Rationale: Mesenchymal stem/stromal cell (MSC)–small extracellular vesicle (MEx) treatment has shown promise in experimental models of neonatal lung injury. The molecular mechanisms by which MEx afford beneficial effects remain incompletely understood.
Objectives: To investigate the therapeutic mechanism of action through assessment of MEx biodistribution and impact on immune cell phenotypic heterogeneity.
Methods: MEx were isolated from the conditioned medium of human umbilical cord Wharton’s jelly–derived MSCs. Newborn mice were exposed to hyperoxia (HYRX, 75% O2) from birth and returned to room air at Postnatal Day 14 (PN14). Mice received either a bolus intravenous MEx dose at PN4 or bone marrow–derived myeloid cells (BMDMy) pretreated with MEx. Animals were killed at PN4, PN7, PN14, or PN28 to characterize MEx biodistribution or for assessment of pulmonary parameters …
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GR Willis, M Reis, AH Gheinani… - American Journal of Respiratory and Critical Care …, 2021