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The aim of the current study was to isolate and investigate one of the most common genera of Gram positive spore forming bacteria, genus Bacillus. Bacillus cereus BC2 was grown well on nutrient agar plates (pH 8) after 24 hrs of incubation at 55oC. This isolate was characterized by producing extracellular protease which showed activity at 55 C both in vivo & in vitro. The optimum enzyme production was at pH 8, incubation temperature 55 C, and NaCl concentration of 5%(w/v) where the highest protein concentration and the highest enzyme activity were recorded. The activity of the partially purified enzyme reached to 160.00 UmL-1 with about 3.10 mg mL-1 protein concentration recorded. The isolate Bacillus cereus BC2 was resistant to the β-Lactam antibiotics tested including ampicillin. Finally, plasmid DNA extraction from the isolate under study and visualization through agarose gel electrophoresis showed the absence of any free plasmid molecules within the vegetative cells of this isolate.[Abdel-Shakour EH and Roushdy MM Plasmid Profile and Protease Activity of β-Lactams Resistant Thermotolerant Soil Isolate B. cereus BC2 from the Bacillus cereus Group Species. Report and Opinion 2010; 2 (8): 82-94].(ISSN: 1553-9873).