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Remote biomass estimation can benefit agricultural practices in several ways, especially larger areas since it does not require local measurements. The advances of the last few decades in machine learning techniques have created new possibilities for estimating aboveground biomass. A pipeline was established from image acquisition to modelling shoot biomass of two wheat cultivars used in Southern Brazil (TBIO Toruk and BRS Parrudo). A UAV was used to acquire multispectral images with high spatial resolution to calculate vegetation indices (VIs). These VIs along with machine learning approaches are used to model the measured biomass of crops in different growth phases. To correlate the wheat images with measured shoot dry biomass, the following regression models were investigated: random forest, support vector regression, and artificial neural networks. An experiment was designed and conducted at the …