作者
Gong‐Hong Wei, Gwenael Badis, Michael F Berger, Teemu Kivioja, Kimmo Palin, Martin Enge, Martin Bonke, Arttu Jolma, Markku Varjosalo, Andrew R Gehrke, Jian Yan, Shaheynoor Talukder, Mikko Turunen, Mikko Taipale, Hendrik G Stunnenberg, Esko Ukkonen, Timothy R Hughes, Martha L Bulyk, Jussi Taipale
发表日期
2010/7/7
期刊
The EMBO journal
卷号
29
期号
13
页码范围
2147-2160
出版商
John Wiley & Sons, Ltd
简介
Members of the large ETS family of transcription factors (TFs) have highly similar DNA‐binding domains (DBDs)—yet they have diverse functions and activities in physiology and oncogenesis. Some differences in DNA‐binding preferences within this family have been described, but they have not been analysed systematically, and their contributions to targeting remain largely uncharacterized. We report here the DNA‐binding profiles for all human and mouse ETS factors, which we generated using two different methods: a high‐throughput microwell‐based TF DNA‐binding specificity assay, and protein‐binding microarrays (PBMs). Both approaches reveal that the ETS‐binding profiles cluster into four distinct classes, and that all ETS factors linked to cancer, ERG, ETV1, ETV4 and FLI1, fall into just one of these classes. We identify amino‐acid residues that are critical for the differences in specificity between all the …
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GH Wei, G Badis, MF Berger, T Kivioja, K Palin, M Enge… - The EMBO journal, 2010