作者
Ximena Camacho, Carolina Perroni, Camila L Machado, Camila de Godoi Carneiro, Mara de Souza Junqueira, Daniele Faria, María F García, Marcelo Fernández, Natalia Oddone, Juan Benech, Carlos A Buchpiguel, Hugo Cerecetto, Roger Chammas, Eloisa Riva, Pablo Cabral, Juan P Gambini
发表日期
2021/9/1
期刊
Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents)
卷号
21
期号
14
页码范围
1883-1893
出版商
Bentham Science Publishers
简介
Background
Multiple Myeloma (MM) is a malignant hematologic disorder and the second most common blood cancer. Interleukin-6 (IL-6) has been identified as a crucial factor for the proliferation and survival of MM cells and the overexpression of IL-6 receptor is being studied as a molecular target for therapeutic and diagnostic use in myelomas and other comorbidities. Tocilizumab is a humanized monoclonal antibody that binds IL-6R.
Objective
We aim to label and evaluate Fab(Tocilizumab) with 99mTechnetium or Cy7 as potential MM imaging agents.
Methods
IL-6R distribution was analyzed by Laser Confocal Microscopy (LCM) in MM cell lines. Fab(Tocilizumab) was produced by the digestion of Tocilizumab with papain for 24h at 37°C, derivatized with NHS-HYNIC-Tfa and radiolabeled with 99mTc. Radiochemical stability and in vitro cell assays were evaluated. Biodistribution and SPECT/CT were performed …
学术搜索中的文章
X Camacho, C Perroni, CL Machado… - Anti-Cancer Agents in Medicinal Chemistry (Formerly …, 2021