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To evaluate the surgical outcome of preserved amniotic membrane transplantation (AMT) for ocular surface reconstruction in chemical burn with limbal stem cell deficiency.

Prospective, noncomparative, interventional case series.

Twenty eyes of 20 consecutive patients with limbal stem cell deficiency secondary to ocular chemical injury.

AMT with or without adjunctive limbal transplantation using limbal tissue from either the healthy contralateral eye (CLAU) or a living related donor (lr-CLAL).

Reconstruction of corneal epithelium (clear appearance without epithelial defect, normal fluorescein permeability and the absence of conjunctiva-derived goblet cells on impression cytology), decrease in corneal vascularization and improvement in visual acuity.

With a mean follow-up time of 19 months (range, 8–27 months …