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Studying the possible association between SNP (rs6631) residing in the miRNA-binding-site of spermatogenesis-related gene (CGA) with azoospermia male infertility in Iranian population was our purpose of this research. CGA has essential role in gonads function. SNP is located in the miRNA-binding site in 3′ untranslated region (3′-UTR) gene may lead to disruption of CGA and elevate the risk of male infertility.

Blood samples were collected from different ethnicities and their DNA was extracted by using salting out method. Sixty-eight infertile men suffer from azoospermia and sixty-three fertile men were genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique and some of them were sequenced randomly. Statistical analyses were performed to investigate the association between rs6631 and azoospermia.

CGA-215AA genotype was …