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β-d-fructofuranosidase is one of the most important enzymes of the food industry especially due to its application in preparation of soft centered candies, confectioneries and to produce fructose syrups. Although several bacteria and filamentous fungi are reported for its production, yeasts are the most preferred source for this enzyme. In the present study mineral nutrients were screened for their effect on yeast growth and the enzyme production using Plackett-Burman design. Of the 11 nutrients screened, three variables (KH₂PO₄, FeCl₃ and CoCl₂) were significantly effecting yeast growth while six variables, i.e. KH₂PO₄, CaCl₂, MnSO₄, Na₂MoO₄, ZnSO₄ and CoCl₂ had significant effect on enzyme production.