发明者
James H Jett, Richard A Keller, John C Martin, Richard G Posner, Babetta L Marrone, Mark L Hammond, Daniel J Simpson
发表日期
1995/4/11
专利局
US
专利号
5405747
专利申请号
08208506
简介
Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.
引用总数
学术搜索中的文章
JH Jett, RA Keller, JC Martin, RG Posner, BL Marrone… - US Patent 5,405,747, 1995