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Objective.To determine whether enhanced daily cleaning would reduce contamination of healthcare worker (HCW) gowns and gloves with methicillin-resistant Staphylococcus aureus (MRSA) or multidrug-resistant Acinetobacter baumannii (MDRAB).Design.A cluster-randomized controlled trial.Setting.Four intensive care units (ICUs) in an urban tertiary care hospital.PARTICIPANTS.ICU rooms occupied by patients colonized with MRSA or MDRAB.INTERVENTION.Extra enhanced daily cleaning of ICU room surfaces frequently touched by HCWs.Results.A total of 4,444 cultures were collected from 132 rooms over 10 months. Using fluorescent dot markers at 2,199 surfaces, we found that 26% of surfaces in control rooms were cleaned and that 100% of surfaces in experimental rooms were cleaned (P < .001). The mean proportion of contaminated HCW gowns and gloves following routine care provision and before …