作者
Bo Xiao, Hamed Laroui, Saravanan Ayyadurai, Emilie Viennois, Moiz A Charania, Yuchen Zhang, Didier Merlin
发表日期
2013/10/1
期刊
Biomaterials
卷号
34
期号
30
页码范围
7471-7482
出版商
Elsevier
简介
The application of RNA interference (RNAi) for inflammatory bowel disease (IBD) therapy has been limited by the lack of non-cytotoxic, efficient and targetable small interfering RNA (siRNA) carriers. TNF-α is the major pro-inflammatory cytokine mainly secreted by macrophages during IBD. Here, a mannosylated bioreducible cationic polymer (PPM) was synthesized and further spontaneously assembled nanoparticles (NPs) assisted by sodium triphosphate (TPP). The TPP-PPM/siRNA NPs exhibited high uniformity (polydispersity index = 0.004), a small particle size (211−275 nm), excellent bioreducibility, and enhanced cellular uptake. Additionally, the generated NPs had negative cytotoxicity compared to control NPs fabricated by branched polyethylenimine (bPEI, 25 kDa) or Oligofectamine (OF) and siRNA. In vitro gene silencing experiments revealed that TPP-PPM/TNF-α siRNA NPs with a weight ratio of 40:1 …
学术搜索中的文章
B Xiao, H Laroui, S Ayyadurai, E Viennois… - Biomaterials, 2013