作者
Alessandra Casagrande Ribeiro, Edimar Aparecida Filomeno Fontes, Camilo José Ramirez López, Wassali Valadares Sousa, Isabela Souza Gomes, Edvaldo Barros, Jane Sélia dos Reis Coimbra, Sylvia do Carmo Castro Franceschini, Danielle Aparecida Silva, Maria Cristina Baracat‐Pereira
发表日期
2023/6
期刊
Molecular Nutrition & Food Research
卷号
67
期号
12
页码范围
2200308
简介
Scope
Human milk (HM) has a wide range of proteins with biological and nutritional functions, essential for newborns. The roles of proteins and their proteoforms in HM are not fully understood. This study aims to assess, by 2‐DE proteomics, the differential proteoforms in HM, present in colostrum (COL), transition (TRA), and mature milk (MAT), aiming to contribute to understanding neonates’ protein needs.
Methods and results
HM samples are collected from 39 healthy lactating women. COL presents the higher concentration of essential amino acids. After MALDI‐MS/MS and bioinformatics analysis, proteoforms are differentially detected. Abundances of β‐casein (CSN2), α‐s1 casein, and α‐lactalbumin (LALBA) are higher in MAT; CSN2s are found in 11 spots and the isoforms increase in size as the pI becomes more acidic; regarding LALBA, two variant forms are found with different abundances in TRA and MAT …
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AC Ribeiro, EAF Fontes, CJR López, WV Sousa… - Molecular Nutrition & Food Research, 2023