作者
Lei Zhu, Venkata G Poosarla, Sooyeon Song, Thammajun L Wood, Daniel S Miller, Bei Yin, Thomas K Wood
发表日期
2018/6
期刊
Environmental microbiology
卷号
20
期号
6
页码范围
2026-2037
简介
Biofilms of sulfate‐reducing bacteria (SRB) produce H2S, which contributes to corrosion. Although bacterial cells in biofilms are cemented together, they often dissolve their own biofilm to allow the cells to disperse. Using Desulfovibrio vulgaris as a model SRB, we sought polysaccharide‐degrading enzymes that disperse its biofilm. Using a whole‐genome approach, we identified eight enzymes as putative extracellular glycoside hydrolases including DisH (DVU2239, dispersal hexosaminidase), an enzyme that we demonstrated here, by utilizing various p‐nitrooligosaccharide substrates, to be an N‐acetyl‐β‐D‐hexosaminidase. For N‐acetyl‐β‐D‐galactosamine (GalNAc), Vmax was 3.6 µmol of p‐nitrophenyl/min (mg protein)−1 and Km was 0.8 mM; the specific activity for N‐acetyl β‐D‐glucosamine (GlcNAc) was 7.8 µmol of p‐nitrophenyl/min (mg protein)−1. Since GalNAc is one of the three exopolysaccharide …
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