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Early embryo development is an exquisitely sensitive period of the developmental continuum, profoundly influencing neonatal and adult health. Maternal and paternal hyperglycaemia is associated with long-term health complications including increased risk of miscarriage, altered birth weight, and increased incidence of diabetes and metabolic syndrome in adult life. The mechanisms underlying this intergenerational transmission of disease remain unclear. New evidence has implicated the ten-eleven-translocation (TET) family of chromatin-modifying enzymes in O-linked glycosyltransferase (OGT)-dependent histone glycosylation. This led us to assess these systems in the mouse oocyte and early embryo. We demonstrated co-precipitation of TET3 (the key TET family member in the oocyte and early embryo) and OGT. In oocytes matured under hyperglycaemic conditions, we identified significant alterations to …