作者
Kylie R Dunning, Michelle Lane, Hannah M Brown, Christine Yeo, Rebecca L Robker, Darryl L Russell
发表日期
2007/11/1
期刊
Human reproduction
卷号
22
期号
11
页码范围
2842-2850
出版商
Oxford University Press
简介
BACKGROUND
In vitro maturation (IVM) of mammalian oocytes has potential health benefits for patients undergoing assisted reproduction as an alternative to gonadotrophin treatment. This procedure is also useful for studying the process of oocyte and early embryo development. However, oocytes undergoing IVM have much lower competence than in vivo matured oocytes. Efforts to optimize IVM success have focused on replicating in vivo timing, hormonal milieu and cumulus cell responses associated with maturing oocytes. We have previously identified two extracellular matrix proteins, the protease Adamts1 and hyaluronan-binding proteoglycan Versican, produced by mural granulosa cells that selectively incorporate into the periovulatory cumulus-oocyte complex (COC).
METHODS
Murine COC were cultured in the presence of epidermal growth factor and/or FSH …
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