作者
Colin W MacDiarmid, Janet Taggart, Jeeyon Jeong, Kittikhun Kerdsomboon, David J Eide
发表日期
2016/9/2
期刊
Journal of Biological Chemistry
卷号
291
期号
36
页码范围
18880-18896
出版商
Elsevier
简介
Stability of many proteins requires zinc. Zinc deficiency disrupts their folding, and the ubiquitin-proteasome system may help manage this stress. In Saccharomyces cerevisiae, UBI4 encodes five tandem ubiquitin monomers and is essential for growth in zinc-deficient conditions. Although UBI4 is only one of four ubiquitin-encoding genes in the genome, a dramatic decrease in ubiquitin was observed in zinc-deficient ubi4Δ cells. The three other ubiquitin genes were strongly repressed under these conditions, contributing to the decline in ubiquitin. In a screen for ubi4Δ suppressors, a hypomorphic allele of the RPT2 proteasome regulatory subunit gene (rpt2E301K) suppressed the ubi4Δ growth defect. The rpt2E301K mutation also increased ubiquitin accumulation in zinc-deficient cells, and by using a ubiquitin-independent proteasome substrate we found that proteasome activity was reduced. These results suggested …
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CW MacDiarmid, J Taggart, J Jeong, K Kerdsomboon… - Journal of Biological Chemistry, 2016