作者
Yoshifumi Takei, Kenji Kadomatsu, Yukio Yuzawa, Seiichi Matsuo, Takashi Muramatsu
发表日期
2004/5/15
期刊
Cancer research
卷号
64
期号
10
页码范围
3365-3370
出版商
American Association for Cancer Research
简介
Vascular endothelial growth factor (VEGF) plays a critical role during normal embryonic angiogenesis and also in the pathological angiogenesis that occurs in a number of diseases, including cancer. We developed a novel VEGF blockade system using RNA interference. The small interfering RNA (siRNA) targeting human VEGF almost completely inhibited the secretion of VEGF in a human prostate cancer cell line, PC-3, whereas the control scramble siRNA showed no effects. The VEGF siRNA with atelocollagen dramatically suppressed tumor angiogenesis and tumor growth in a PC-3 s.c. xenograft model. Atelocollagen provided a beneficial delivering means by which stabilization and efficient transfection of the siRNA injected into the tumors were achieved.
引用总数
学术搜索中的文章
Y Takei, K Kadomatsu, Y Yuzawa, S Matsuo… - Cancer research, 2004