作者
Vânia Cristina Desoti, Danielle Lazarin-Bidóia, Daniela Bueno Sudatti, Renato Crespo Pereira, Antonio Alonso, Tania Ueda-Nakamura, Benedito Prado Dias Filho, Celso Vataru Nakamura, Sueli de Oliveira Silva
发表日期
2012/8/3
期刊
Marine Drugs
卷号
10
期号
8
页码范围
1631-1646
出版商
MDPI
简介
Natural compounds have shown good potential for the discovery of new chemotherapeutics for the treatment of Chagas’ disease. Recently, our group reported the effective trypanocidal activity of (−)-elatol, extracted from the red macroalgae Laurencia dendroidea present in the Brazilian coast against Trypanosoma cruzi. However, the mechanism of action of this compound has remained unclear. There are only hypotheses concerning its action on mitochondrial function. Here, we further investigated the mechanisms of action of (−)-elatol on trypomastigotes of T. cruzi. For this, we evaluated some biochemical alterations in trypomastigotes treated with (−)-elatol. Our results show that (−)-elatol induced depolarization of the mitochondrial membrane, an increase in the formation of mitochondrial superoxide anion and loss of cell membrane and DNA integrity. Additionally, (−)-elatol induced formation of autophagic vacuoles and a decrease in cell volume. All together, these results suggest that the trypanocidal action of (−)-elatol involves multiple events and mitochondria might be the initial target organelle. Our hypothesis is that the mitochondrial dysfunction leads to an increase of ROS production through the electron transport chain, which affects cell membrane and DNA integrity leading to different types of parasite death.
学术搜索中的文章
Trypanocidal action of (−)-elatol involves an oxidative stress triggered by mitochondria dysfunction
VC Desoti, D Lazarin-Bidóia, DB Sudatti, RC Pereira… - Marine Drugs, 2012