作者
David Pamies, Paula Barrera, Katharina Block, Georgia Makri, Anupama Kumar, Daphne Wiersma, Lena Smirnova, Ce Zhang, Joseph Bressler, Kimberly M Christian, Georgina Harris, Guo-li Ming, Cindy J Berlinicke, Kelly Kyro, Hongjun Song, Carlos A Pardo, Thomas Hartung, Helena T Hogberg
发表日期
2017
期刊
Altex
卷号
34
期号
3
页码范围
362
出版商
NIH Public Access
简介
Human in vitro models of brain neurophysiology are needed to investigate molecular and cellular mechanisms associated with neurological disorders and neurotoxicity. We have developed a reproducible iPSC-derived human 3D brain microphysiological system (BMPS), comprised of differentiated mature neurons and glial cells (astrocytes and oligodendrocytes) that reproduce neuronal-glial interactions and connectivity. BMPS mature over eight weeks and show the critical elements of neuronal function: synaptogenesis and neuron-to-neuron (eg, spontaneous electric field potentials) and neuronal-glial interactions (eg, myelination), which mimic the microenvironment of the central nervous system, rarely seen in vitro before. The BMPS shows 40% overall myelination after 8 weeks of differentiation. Myelin was observed by immunohistochemistry and confirmed by confocal microscopy 3D reconstruction and electron …
学术搜索中的文章
D Pamies, P Barrera, K Block, G Makri, A Kumar… - Altex, 2017