作者
Catarina Pinho, Sara Rocha, Bruno M Carvalho, Susana Lopes, Sofia Mourao, Marcelo Vallinoto, Tuliana O Brunes, Célio FB Haddad, Helena Gonçalves, Fernando Sequeira, Nuno Ferrand
发表日期
2010/9/1
期刊
Conservation Genetics Resources
卷号
2
期号
1
页码范围
181-185
出版商
Springer Netherlands
简介
We report new primers for the amplification and sequencing of 11 nuclear markers in squamate reptiles and anuran amphibians (five in squamates, six in anurans). Ten out of the 11 loci are introns (three of which are linked) that were amplified using an exon-primed, intron-crossing (EPIC) PCR strategy, whereas an eleventh locus spans part of a protein-coding gene. Squamate and anuran primers were initially developed for Lacerta schreiberi (Squamata: Lacertidae) and Pelodytes spp. (Anura: Pelodytidae), respectively. Cross-species amplification of the squamate markers was evaluated in four genera representing two additional families, whereas for anurans three genera corresponding to three additional families were tested. Three out of the five loci were successfully sequenced in all squamate taxa tested. Cross-amplification of the six anuran markers had lower, but still significant, success. We predict …
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