作者
Ozlem Yaren, Jacquelyn McCarter, Andrew R Morris, Kevin M Bradley, Nilesh B Karalkar, Daniel C McLendon, Dongmin Kim, Bradley H Eastmond, Nathan D Burkett-Cadena, Barry W Alto, Steven A Benner
发表日期
2023/6/30
期刊
Analytical Chemistry
卷号
95
期号
28
页码范围
10736-10743
出版商
American Chemical Society
简介
Recently reported “displaceable probe” loop amplification (DP-LAMP) architecture has shown to amplify viral RNA from SARS-CoV-2 with little sample processing. The architecture allows signals indicating the presence of target nucleic acids to be spatially separated, and independent in sequence, from the complicated concatemer that LAMP processes create as part of their amplification process. This makes DP-LAMP an attractive molecular strategy to integrate with trap and sampling innovations to detect RNA from arboviruses carried by mosquitoes in the field. These innovations include (a) development of organically produced carbon dioxide with ethylene carbonate as a bait deployable in mosquito trap, avoiding the need for dry ice, propane tanks, or inorganic carbonates and (b) a process that induces mosquitoes to lay virus-infected saliva on a quaternary ammonium-functionalized paper (Q-paper) matrix …
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