作者
Lin Cong, Yi-Ping Xia, Gui-Qiu Zhao, Jing Lin, Qiang Xu, Li-Ting Hu, Jian-Qiu Qu, Xu-Dong Peng
发表日期
2015
期刊
International Journal of Ophthalmology
卷号
8
期号
5
页码范围
866
出版商
Press of International Journal of Ophthalmology
简介
AIM
To observe the expression of vitamin D receptor (VDR) in human specimen and immortalized human corneal epithelium cells (HCEC) when challenged with fusarium solani. Moreover, we decided to discover the pathway of VDR expression. Also, we would like to detect the expression of cathelicidin antimicrobial peptide (CAMP) in the downstream pathway of VDR.
METHODS
Immunohistochemistry was used to examine the VDR expression in HCEC from healthy and fungal keratitis patients. Real time quantitative polymerase chain reaction (qPCR) was performed to observe the messenger ribonucleic acid (mRNA) change of VDR when immortalized HCEC were challenged with fusarium solani for different hours. CAMP was detected at both mRNA and protein levels.
RESULTS
We found out that the VDR expression in fusarium solani keratitis patients' specimen was much more than that in healthy people. The …
学术搜索中的文章
L Cong, YP Xia, GQ Zhao, J Lin, Q Xu, LT Hu, JQ Qu… - International Journal of Ophthalmology, 2015