作者
Alwine A Hellingman, Antonius JNM Bastiaansen, Margreet R de Vries, Leonard Seghers, Maarten A Lijkwan, Clemens W Löwik, Jaap F Hamming, Paul HA Quax
发表日期
2010/12/1
期刊
European Journal of Vascular and Endovascular Surgery
卷号
40
期号
6
页码范围
796-803
出版商
WB Saunders
简介
OBJECTIVE
To identify the optimal mouse model for hind limb ischaemia, which offers a therapeutic window that is large enough to detect improvements of blood flow recovery, for example, using cell therapies.
MATERIALS AND METHODS
Different surgical approaches were performed: single coagulation of femoral and iliac artery, total excision of femoral artery and double coagulation of femoral and iliac artery. Blood flow restoration was analysed with Laser Doppler Perfusion Imaging (LDPI). Immuno-histochemical stainings, angiography and micro-computed tomography (CT) scans were performed for visualisation of collaterals in the mouse.
RESULTS
Significant differences in flow restoration were observed depending on the surgical procedure. After single coagulation, blood flow already restored 100% in 7 days, in contrast to a significant delayed flow restoration after double coagulation (54% after 28 days, P …
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