作者
Sabine MJ Welten, Antonius JNM Bastiaansen, Rob CM de Jong, Margreet R de Vries, Erna AB Peters, Martin C Boonstra, Søren P Sheikh, Nicola La Monica, Ekambar R Kandimalla, Paul HA Quax, A Yaël Nossent
发表日期
2014/9/26
期刊
Circulation research
卷号
115
期号
8
页码范围
696-708
出版商
Lippincott Williams & Wilkins
简介
Rationale:
Effective neovascularization is crucial for recovery after cardiovascular events.
Objective:
Because microRNAs regulate expression of up to several hundred target genes, we set out to identify microRNAs that target genes in all pathways of the multifactorial neovascularization process. Using www.targetscan.org, we performed a reverse target prediction analysis on a set of 197 genes involved in neovascularization. We found enrichment of binding sites for 27 microRNAs in a single microRNA gene cluster. Microarray analyses showed upregulation of 14q32 microRNAs during neovascularization in mice after single femoral artery ligation.
Methods and Results:
Gene silencing oligonucleotides (GSOs) were used to inhibit 4 14q32 microRNAs, miR-329, miR-487b, miR-494, and miR-495, 1 day before double femoral artery ligation. Blood flow recovery was followed by laser Doppler perfusion imaging. All 4 …
学术搜索中的文章
SMJ Welten, AJNM Bastiaansen, RCM de Jong… - Circulation research, 2014