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The objective of this work is to prepare and verify the antimicrobial properties of tissue conditioner containing silver doped bioactive glass nanoparticles (Ag-BG NPs) in vitro against three oral pathogens. A novel bioactive glass (BG) nanoparticles (NPs) with different Ag weight percentages of 0.6, 1.2, 3 and 4 wt% was prepared via a modified sol-gel method. Characterization of Ag-BG NPs was carried out using transmission electron microscopy (TEM), Fourier transformer infrared spectrophotometer (FTIR). Antimicrobial activities of the NPs were estimated. Also, ions release was measured using an inductively coupled plasma optical emission spectroscopy (ICP/OES). The 4% Ag-BG NPs was incorporated into the tissue conditioner (TC) for the preparation of the composite specimens (TC/Ag BG 4%). The dispersion of the NPs was evaluated using SEM/EDX. ion release and antimicrobial activities were evaluated …