作者
Ahmed N Hegazy, Nathaniel R West, Michael JT Stubbington, Emily Wendt, Kim IM Suijker, Angeliki Datsi, Sebastien This, Camille Danne, Suzanne Campion, Sylvia H Duncan, Benjamin MJ Owens, Holm H Uhlig, Andrew McMichael, Andreas Bergthaler, Sarah A Teichmann, Satish Keshav, Fiona Powrie, Oxford IBD Cohort Investigators
发表日期
2017/11/1
期刊
Gastroenterology
卷号
153
期号
5
页码范围
1320-1337. e16
出版商
WB Saunders
简介
Background & Aims
Interactions between commensal microbes and the immune system are tightly regulated and maintain intestinal homeostasis, but little is known about these interactions in humans. We investigated responses of human CD4+ T cells to the intestinal microbiota. We measured the abundance of T cells in circulation and intestinal tissues that respond to intestinal microbes and determined their clonal diversity. We also assessed their functional phenotypes and effects on intestinal resident cell populations, and studied alterations in microbe-reactive T cells in patients with chronic intestinal inflammation.
Methods
We collected samples of peripheral blood mononuclear cells and intestinal tissues from healthy individuals (controls, n = 13−30) and patients with inflammatory bowel diseases (n = 119; 59 with ulcerative colitis and 60 with Crohn’s disease). We used 2 independent assays (CD154 detection and …
学术搜索中的文章
AN Hegazy, NR West, MJT Stubbington, E Wendt… - Gastroenterology, 2017