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Background: The clinical evaluation of captive large felids has been a challenge for veterinarians and the diversity of management of this animals can complicate the standardization of hematologic parameters to these species. Red blood cells (RBC), white blood cells (WBC), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and platelet counts were measured, as well as plasma fibrinogen levels, from captive lions and Siberian tigers in Brazil. The objective of this study is to provide veterinarians an additional source of information concerning the reference values of hematologic parameters of lions and Siberian tigers.