作者
Joanna Stanczyk, Caroline Ospelt, Emmanuel Karouzakis, Andrew Filer, Karim Raza, Christoph Kolling, Renate Gay, Christopher D Buckley, Paul P Tak, Steffen Gay, Diego Kyburz
发表日期
2011/2
期刊
Arthritis & Rheumatism
卷号
63
期号
2
页码范围
373-381
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
Objective
MicroRNA (miRNA) are recognized as important regulators of a variety of fundamental biologic processes. Previously, we described increased expression of miR‐155 and miR‐146a in rheumatoid arthritis (RA) and showed a repressive effect of miR‐155 on matrix metalloproteinase (MMP) expression in RA synovial fibroblasts (RASFs). The present study was undertaken to examine alterations in expression of miR‐203 in RASFs and analyze its role in fibroblast activation.
Methods
Differentially expressed miRNA in RASFs versus osteoarthritis synovial fibroblasts (OASFs) were identified by real‐time polymerase chain reaction (PCR)–based screening of 260 individual miRNA. Transfection of miR‐203 precursor was used to analyze the function of miR‐203 in RASFs. Levels of interleukin‐6 (IL‐6) and MMPs were measured by real‐time PCR and enzyme‐linked immunosorbent assay. RASFs were …
学术搜索中的文章
J Stanczyk, C Ospelt, E Karouzakis, A Filer, K Raza… - Arthritis & Rheumatism, 2011