作者
C Ospelt, A Engler, P Künzler, RE Gay, BA Michel, S Gay
发表日期
2013/6/1
期刊
Annals of the Rheumatic Diseases
卷号
72
期号
Suppl 3
页码范围
A794-A794
出版商
BMJ Publishing Group Ltd
简介
Background
Even though it is well known that smokers have an increased risk to develop rheumatoid arthritis (RA) and other autoimmune diseases, nothing is known about the molecular mechanisms that lead to break of tolerance in smokers. The innate immune receptor NKG2D and its ligands MICA and MICB have previously been described to promote autoimmune reactions. Therefore, we analysed whether smoking leads to increased expression of NKG2D ligands and activates the NKG2D system.
Methods
RA and osteoarthritis (OA) synovial fibroblasts (SFs) were cultured from synovial tissues from patients undergoing joint replacement surgery. Real-time PCR was used to measure expression levels of NKG2D ligands in synovial tissues and SFs stimulated with 5% cigarette smoke extract (CSE). Peripheral blood mononuclear cells (PBMCs) were isolated from healthy volunteers and co-cultured with RASFs …
学术搜索中的文章
C Ospelt, A Engler, P Künzler, RE Gay, BA Michel… - Annals of the Rheumatic Diseases, 2013