作者
Mária Filková, Borbala Aradi, Ladislav Šenolt, Caroline Ospelt, Serena Vettori, Heřman Mann, Andrew Filer, Karim Raza, Christopher D Buckley, Martyn Snow, Jiří Vencovský, Karel Pavelka, Beat A Michel, Renate E Gay, Steffen Gay, Astrid Jüngel
发表日期
2014/10/1
期刊
Annals of the rheumatic diseases
卷号
73
期号
10
页码范围
1898-1904
出版商
BMJ Publishing Group Ltd
简介
Background
Identification of parameters for early diagnosis and treatment response would be beneficial for patients with early rheumatoid arthritis (ERA) to prevent ongoing joint damage. miRNAs have features of potential biomarkers, and an altered expression of miRNAs was shown in established rheumatoid arthritis (RA).
Objective
To analyse RA associated miRNAs in the sera of patients with ERA to find markers of early disease, clinical activity or predictors of disease outcome.
Methods
Total RNA was isolated from whole sera in ERA patients (prior to and after 3 and 12 months of therapy with disease modifying antirheumatic drugs), in patients with established RA and in healthy controls (HC) using phenol–chloroform extraction. Expression of miR-146a, miR-155, miR-223, miR-16, miR-203, miR-132 and miR-124a was analysed by TaqMan Real Time PCR.
Results
From all analysed miRNAs, levels of miR …
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