作者
Fabienne Niederer, Michelle Trenkmann, Caroline Ospelt, Emmanuel Karouzakis, Michel Neidhart, Joanna Stanczyk, Christoph Kolling, Renate E Gay, Michael Detmar, Steffen Gay, Astrid Jüngel, Diego Kyburz
发表日期
2012/6
期刊
Arthritis & Rheumatism
卷号
64
期号
6
页码范围
1771-1779
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
Objective
To investigate the expression and effect of the microRNA‐34 (miR‐34) family on apoptosis in rheumatoid arthritis synovial fibroblasts (RASFs).
Methods
Expression of the miR‐34 family in synovial fibroblasts with or without stimulation with Toll‐like receptor (TLR) ligands, tumor necrosis factor α (TNFα), interleukin‐1β (IL‐1β), hypoxia, or 5‐azacytidine was analyzed by real‐time polymerase chain reaction (PCR). Promoter methylation was studied by combined bisulfite restriction analysis. The effects of overexpression and silencing of miR‐34a and miR‐34a* on apoptosis were analyzed by annexin V/propidium iodide staining. Production of X‐linked inhibitor of apoptosis protein (XIAP) was assessed by real‐time PCR and immunohistochemistry analysis. Reporter gene assay was used to study the signaling pathways of miR‐34a*.
Results
Basal expression levels of miR‐34a* were found to be reduced …
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F Niederer, M Trenkmann, C Ospelt, E Karouzakis… - Arthritis & Rheumatism, 2012