作者
Caroline Ospelt, Fabia Brentano, Yvonne Rengel, Joanna Stanczyk, Christoph Kolling, Paul P Tak, Renate E Gay, Steffen Gay, Diego Kyburz
发表日期
2008/12
期刊
Arthritis & Rheumatism: Official Journal of the American College of Rheumatology
卷号
58
期号
12
页码范围
3684-3692
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
Objective
To analyze the expression, regulation, and biologic relevance of Toll‐like receptors (TLRs) 1–10 in synovial and skin fibroblasts and to determine the expression levels of TLRs 2, 3, and 4 in synovial tissues from patients with early rheumatoid arthritis (RA), longstanding RA, and osteoarthritis (OA).
Methods
Expression of TLRs 1–10 in RA synovial fibroblasts (RASFs), OASFs, and skin fibroblasts was analyzed by real‐time polymerase chain reaction (PCR). Fibroblasts were stimulated with tumor necrosis factor α, interleukin‐1β (IL‐1β), bacterial lipopeptide, poly(I‐C), lipopolysaccharide, and flagellin. Production of IL‐6 was determined by enzyme‐linked immunosorbent assay and induction of TLRs 2–5, matrix metalloproteinases (MMPs) 3 and 13 messenger RNA by real‐time PCR. Expression of TLRs 2–4 in synovial tissues was analyzed by immunohistochemistry.
Results
Synovial fibroblasts …
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