作者
Colleen Milroy, Lihua Liu, Sue Hammoud, Ahmad Hammoud, C Matthew Peterson, Douglas T Carrell
发表日期
2011/5/1
期刊
Fertility and sterility
卷号
95
期号
6
页码范围
2094-2099
出版商
Elsevier
简介
Objective
To assess the methylation patterns of four pluripotency gene promoters in mouse oocytes after in vivo maturation, in vitro maturation (IVM), and vitrification followed by IVM.
Design
Experimental study.
Setting
Research laboratory.
Animal(s)
Three populations of metaphase II mouse oocytes were analyzed after in vivo maturation, IVM, and vitrification followed by IVM (V-IVM). Cumulus cells and blastocyst embryos were controls.
Intervention(s)
The CpG methylation patterns (overall and CpG specific) in the promoters of four pluripotency genes (Oct4, Nanog, Foxd3, and Sox2) were analyzed for each cell type by traditional DNA bisulfite sequencing.
Main Outcome Measure(s)
Differences for overall methylation were evaluated using the Student’s t-test and for individual CpG sites by χ2 analysis.
Result(s)
Significantly lower levels of overall methylation in promoters of Oct4 (25%) and Sox2 (4.5%) were noted in V-IVM …
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