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The Murucupi River forms an important tributary of the Marajó bay, within complex basin of the Amazon region, where the local population uses the river water as a food source (fish) and transport. In April 2009, the Murucupi River received a large amount of toxic waste that leaked from containment basins of red mud of the industrial hub in Barcarena, where does the processing of bauxite to produce alumina by the Bayer process. After pouring, the biota of the Murucupi River was completely affected, leading to death of fish, plants and microorganisms. Whereas the Murucupi River suffers direct influence of the tide that enters in his bed, this study aims to evaluate from studies of seasonal chemical and physical parameters of metal concentrations in the Murucupi riverbed, the reestablishment of the environmental balance by applying the statistical model to interpret the results.