作者
Masood Abu-Halima, Zyiad Abu Khaizaran, Basim M Ayesh, Ulrike Fischer, Salem Abu Khaizaran, Feras Al-Battah, Mohamad Hammadeh, Andreas Keller, Eckart Meese
发表日期
2020/5/1
期刊
Fertility and sterility
卷号
113
期号
5
页码范围
970-980. e2
出版商
Elsevier
简介
Objective
To identify differentially abundant miRNAs in sperm samples and spent culture media (SCM) of embryos of different grade toward a prediction of pregnancy outcome.
Design
Array-based reverse-transcription quantitative polymerase chain reaction profiling and validation.
Setting
University research institute and in vitro fertilization center.
Patient(s)
Couples (n = 61) undergoing infertility treatment with the use of intracytoplasmic sperm injection.
Interventions(s)
None.
Main Outcome Measure(s)
Abundance levels of miRNAs in combined SCM of embryos of different quality and in sperm samples associated with pregnancy outcome.
Result(s)
Out of 372 screened miRNAs, miR-19b-3p and let-7a-5p were detected consistently in all SCM and sperm samples. The abundance levels of miRNAs were significantly altered between SCM of embryos with different quality (G1, G2, and G3 grades). Specifically, miR-320a and …
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M Abu-Halima, ZA Khaizaran, BM Ayesh, U Fischer… - Fertility and sterility, 2020