查看文章

VIP inhibits long-term potentiation in the CA1 area of the rat hippocampus through activation of VPAC1 receptors (1). VIP release is triggered by electrical activity and VIP receptors are enhanced in TLE patients (2). In this work we investigated if VPAC1 receptor activation during in vitro interictal-like activity (ILA), such as the observed during epileptogenesis, could influence the subsequent expression of LTP and GABAergic, glutamatergic and lipid raft synaptic markers in the CA1 area of the hippocampus. Male wistar rats (6-7 week-old) were decapitated under fluothane anaesthesia. Extracellular electrophysiological recordings of field-excitatory post-synaptic potentials (fEPSPs) evoked by electrical stimulation in hippocampal slices were used to access synaptic transmission. In vitro ILA was induced by 30 min superfusion with aCSF containing 0mM MgCl2 and 6mM KCl (in normal comp.: 1mM MgCl2 and 3mM KCl …