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Objective: To investigate the occurrence of Campylobacter jejuni among other bacterial agents of acute diarrhea. Subjects and Methods: Over a period of 3 months, 114 specimens of acute diarrhea were collected from patients aged 2–58 years referred to Nemazi Hospital, Shiraz, Iran. The samples were tested for bacterial pathogens causing acute diarrhea. Results: Of the 114 samples, 40 proved to be positive for bacterial enteric pathogens: C. jejuni 11 (9.6%), Salmonella spp.18 (15.8%) and Shigella spp. 11 (9.6%). Maximum prevalence of C. jejuni was observed in patients aged 11–15 years. Significantly more females than males were infected with all diarrheal agents (p <0.05). Conclusion: This study confirmed the presence of C. jejuni as a significant cause of gastroenteritis in Shiraz, Southwest Iran.