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Background

The objective of the present study was to select combinations of primers for genetic polymorphisms (INDELS) used in the analysis of chimerism associated with post-transplant hematopoietic regeneration as well as to test them under real-time PCR (qPCR) and droplet digital PCR (ddPCR) conditions.

Methods

We searched for genetic polymorphisms, using the EMBL-European Molecular Biology Laboratory-database taking into account certain criteria for selection. There were 85 polymorphisms left at the end of the selection process. For both techniques, we designed primers with the following characteristics: 65 C annealing temperature, amplicon length below 120 bp, at least 3 different nucleotides at the 3’end between variants and temperature difference between forward and reverse primer of maximum 1 C. Depending on the distribution of genotypes, assays were designed for one or both alleles …