作者
Futoshi Arakane, Caleb B Kallen, Hidemichi Watari, James A Foster, Naresh Babu V Sepuri, Debkumar Pain, Steven E Stayrook, Mitchell Lewis, George L Gerton, Jerome F Strauss
发表日期
1998/6/26
期刊
Journal of Biological Chemistry
卷号
273
期号
26
页码范围
16339-16345
出版商
American Society for Biochemistry and Molecular Biology
简介
Steroidogenic acute regulatory protein (StAR) plays an essential role in steroidogenesis, facilitating delivery of cholesterol to cytochrome P450scc on the inner mitochondrial membrane. StAR is synthesized in the cytoplasm and is subsequently imported by mitochondria and processed to a mature form by cleavage of the NH2-terminal mitochondrial targeting sequence. To explore the mechanism of StAR action, we produced 6-histidine-tagged N-62 StAR (His-tag StAR) constructs lacking the NH2-terminal 62 amino acids that encode the mitochondrial targeting sequence and examined their steroidogenic activity in intact cells and on isolated mitochondria. His-tag StAR proteins stimulated pregnenolone synthesis to the same extent as wild-type StAR when expressed in COS-1 cells transfected with the cholesterol side-chain cleavage system. His-tag StAR was diffusely distributed in the cytoplasm of transfected COS …
引用总数
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F Arakane, CB Kallen, H Watari, JA Foster, NBV Sepuri… - Journal of Biological Chemistry, 1998