作者
Jie Li, Mikhail Kogan, Simon AB Knight, Debkumar Pain, Andrew Dancis
发表日期
1999/11/12
期刊
Journal of Biological Chemistry
卷号
274
期号
46
页码范围
33025-33034
出版商
American Society for Biochemistry and Molecular Biology
简介
Nfs1p is the yeast homolog of the bacterial proteins NifS and IscS, enzymes that release sulfur from cysteine for iron-sulfur cluster assembly. Here we show that the yeast mitochondrial protein Nfs1p regulates cellular and mitochondrial iron homeostasis. A strain of Saccharomyces cerevisiae, MA14, with a missenseNFS1 allele (I191S) was isolated in a screen for altered iron-dependent gene regulation. This mutant exhibited constitutive up-regulation of the genes of the cellular iron uptake system, mediated through effects on the Aft1p iron-regulatory protein. Iron accumulating in the mutant cells was retained in the mitochondrial matrix while, at the same time, iron-sulfur proteins were deficient. In this work, the yeast protein was localized to mitochondria, and the gene was shown to be essential for viability. Furthermore, Nfs1p in the MA14 mutant was found to be markedly decreased, suggesting that this low protein …
引用总数
学术搜索中的文章
J Li, M Kogan, SAB Knight, D Pain, A Dancis - Journal of Biological Chemistry, 1999