作者
Debkumar Pain, Hiroshi Murakami, Günter Blobel
发表日期
1990/10/4
期刊
Nature
卷号
347
期号
6292
页码范围
444-449
出版商
Nature Publishing Group
简介
Anti-idiotypic antibodies, prepared using a chemically synthesized signal peptide of a mitochondrial precursor protein, recognized a mitochondrial integral membrane protein (p32). Fab fragments derived from both anti-idiotypic antibodies and monospecific antibodies against purified p32 inhibited protein import into mitochondria. Moreover, anti-p32 antibodies specifically immunoprecipitated a precursor–p32 complex after detergent solubilization of mitochondria. Immunoelectron microscopy and subfractionation of mitochondria indicate that p32 is located in contact sites between the outer and inner mitochondrial membranes.
引用总数
学术搜索中的文章
D Pain, H Murakami, G Blobel - Nature, 1990