作者
Jie Li, Sandeep Saxena, Debkumar Pain, Andrew Dancis
发表日期
2001/1/12
期刊
Journal of Biological Chemistry
卷号
276
期号
2
页码范围
1503-1509
出版商
American Society for Biochemistry and Molecular Biology
简介
Arh1p is an essential mitochondrial protein of yeast with reductase activity. Here we show that this protein is involved in iron metabolism. A yeast strain was constructed in which the open reading frame was placed under the control of a galactose-regulated promoter. Protein expression was induced by galactose and repressed to undetectable levels in the absence of galactose, although cells grew quite well in the absence of inducer. Under noninducing conditions, cellular iron uptake was dysregulated, exhibiting a failure to repress in response to medium iron. Iron trafficking within the cell was also disturbed. Exposure of Arh1p-depleted cells to increasing iron concentrations during growth led to drastic increases in mitochondrial iron, indicating a loss of homeostatic control. Activity of aconitase, a prototype Fe-S protein, was deficient at all concentrations of mitochondrial iron, although the protein level was unaltered …
引用总数
学术搜索中的文章
J Li, S Saxena, D Pain, A Dancis - Journal of Biological Chemistry, 2001