作者
Hiroshi Murakami, Debkumar Pain, Giinter Blobel
发表日期
1988/12/1
期刊
The Journal of cell biology
卷号
107
期号
6
页码范围
2051-2057
出版商
Rockefeller Univ Press
简介
We have developed an in vitro system in which the posttranslational import of Put2 (delta-pyrroline-5-carboxylate dehydrogenase), into yeast mitochondria is dependent on the addition of yeast postribosomal supernatant (PRS). When mRNA for a nuclear-encoded yeast mitochondrial matrix protein, Put2, was translated in a wheat germ cell-free system, import into posttranslationally added yeast mitochondria was negligible. However, when a yeast PRS was added, significant import was observed. The import stimulating activity of the yeast PRS was shown to consist of at least two distinct factors. One of these is the recently purified 70-kD heat shock-related protein Ssalp/Ssa2p, two proteins that are 98% homologous. The other factor is an N-ethylmaleimide-sensitive protein(s). Both factors act synergistically.
引用总数
学术搜索中的文章
H Murakami, D Pain, G Blobel - The Journal of cell biology, 1988