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Isoprene is a substantial contributor to global secondary organic aerosol (SOA). The formation of isoprene SOA (SOA_I) is highly influenced by anthropogenic emissions. Currently, there is rare information regarding SOA_I in polluted regions. In this study, one-year concurrent observation of SOA_I tracers was undertaken at 12 sites across China for the first time. The tracers formed from the HO₂-channel exhibited higher concentrations at rural sites, while the tracer formed from the NO/NO₂-channel showed higher levels at urban sites. 3-Methyltetrahydrofuran-3,4-diols exhibited linear correlations with their ring-opening products, C₅-alkenetriols. And the slopes were steeper in the southern China than the northern China, indicating stronger ring-opening reactions there. The correlation analysis of SOA_I tracers with the factor determining biogenic emission and the tracer of biomass burning (levoglucosan) implied that the …