作者
Nancy L Oleinick, Tithi Biswas, Rutulkumar Patel, Mingfang Tao, Ravi Patel, Lachelle Weeks, Neelesh Sharma, Afshin Dowlati, Stanton L Gerson, Pingfu Fu, Junran Zhang, Mitchell Machtay
发表日期
2016/11/1
期刊
Radiotherapy and Oncology
卷号
121
期号
2
页码范围
335-341
出版商
Elsevier
简介
Background and purpose
The anti-folate pemetrexed is a radiosensitizer. In pre-clinical models, pemetrexed is more effective along with the base-excision-repair inhibitor methoxyamine. We tested whether methoxyamine enhances pemetrexed-mediated radiosensitization of lung adenocarcinoma cells and xenografts.
Materials and methods
A549 and H1299 cells were evaluated for cell cycle distribution by flow cytometry, radiosensitization by clonogenic assay, and DNA repair by neutral comet assay and repair protein activation. H460 cells were included in some studies. Xenografts in nude mice received drug(s) and/or radiation, and tumor growth was monitored by caliper and in vivo toxicity by animal weight.
Results
Exposure to pemetrexed/methoxyamine for 24 (H1299, H460) or 48 (A549) hours before irradiation resulted in accumulation of cells near the radiosensitive G1/S border; dose-enhancement factors of 1 …
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NL Oleinick, T Biswas, R Patel, M Tao, R Patel… - Radiotherapy and Oncology, 2016