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Cerebellar granule neurons possess a non-inactivating K⁺ current, which controls resting membrane potentials and modulates the firing rate by means of muscarinic agonists. kcr1 was cloned from the cerebellar cDNA library by suppression cloning. KCR1 is a novel protein with 12 putative transmembrane domains and enhances the functional expression of the cerebellar non-inactivating K⁺ current in Xenopus oocytes. KCR1 also accelerates the activation of rat EAG K⁺ channels expressed in Xenopusoocytes or in COS-7 cells. Far-Western blotting revealed that KCR1 and EAG proteins interacted with each other by means of their C-terminal regions. These results suggest that KCR1 is the regulatory component of non-inactivating K⁺ channels.