作者
Jayant Asthana, Anuradha Kuchibhatla, Swadhin Chandra Jana, Krishanu Ray, Dulal Panda
发表日期
2012/11/23
期刊
Journal of Biological Chemistry
卷号
287
期号
48
页码范围
40793-40805
出版商
American Society for Biochemistry and Molecular Biology
简介
Dynein light chain 1 (LC8), a highly conserved protein, is known to bind to a variety of different polypeptides. It functions as a dimer, which is inactivated through phosphorylation at the Ser-88 residue. A loss of LC8 function causes apoptosis in Drosophila embryos, and its overexpression induces malignant transformation of breast cancer cells. Here we show that LC8 binds to tubulin, promotes microtubule assembly, and induces the bundling of reconstituted microtubules in vitro. Furthermore, LC8 decorates microtubules both in Drosophila embryos and in HeLa cells, increases the microtubule stability, and promotes microtubule bundling in these cells. Microtubule stability influences a number of different cellular functions including mitosis and cell differentiation. The LC8 overexpression reduces the susceptibility of microtubules to cold and nocodazole-induced depolymerization in tissue-cultured cells and increases …
学术搜索中的文章
J Asthana, A Kuchibhatla, SC Jana, K Ray, D Panda - Journal of biological chemistry, 2012