作者
Carla AM Lopes, Swadhin Chandra Jana, Inês Cunha-Ferreira, Sihem Zitouni, Inês Bento, Paulo Duarte, Samuel Gilberto, Francisco Freixo, Adán Guerrero, Maria Francia, Mariana Lince-Faria, Jorge Carneiro, Mónica Bettencourt-Dias
发表日期
2015/10/26
期刊
Developmental cell
卷号
35
期号
2
页码范围
222-235
出版商
Cell Press
简介
Centrioles are essential for cilia and centrosome assembly. In centriole-containing cells, centrioles always form juxtaposed to pre-existing ones, motivating a century-old debate on centriole biogenesis control. Here, we show that trans-autoactivation of Polo-like kinase 4 (PLK4), the trigger of centriole biogenesis, is a critical event in the spatial control of that process. We demonstrate that centrioles promote PLK4 activation through its recruitment and local accumulation. Though centriole removal reduces the proportion of active PLK4, this is rescued by concentrating PLK4 to the peroxisome lumen. Moreover, while mild overexpression of PLK4 only triggers centriole amplification at the existing centriole, higher PLK4 levels trigger both centriolar and cytoplasmatic (de novo) biogenesis. Hence, centrioles promote their assembly locally and disfavor de novo synthesis. Similar mechanisms enforcing the local …
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CAM Lopes, SC Jana, I Cunha-Ferreira, S Zitouni… - Developmental cell, 2015