作者
Indranil Mitra, Alexey V Nefedov, Allan R Brasier, Rovshan G Sadygov
发表日期
2012/2/22
期刊
Analytical Chemistry
出版商
American Chemical Society
简介
Improvements in the mass accuracy and resolution of mass spectrometers have greatly aided mass spectrometry-based proteomics in profiling complex biological mixtures. With the use of innovative bioinformatics approaches, high mass accuracy and resolution information can be used for filtering chemical noise in mass spectral data. Using our recent algorithmic developments, we have generated the mass distributions of all theoretical tryptic peptides composed of 20 natural amino acids and with masses limited to 3.5 kDa. Peptide masses are distributed discretely, with well-defined peak clusters separated by empty or sparsely populated trough regions. Accurate models for peak centers and widths can be used to filter peptide signals from chemical noise. We modeled mass defects, the difference between monoisotopic and nominal masses, and peak centers and widths in the peptide mass distributions. We found …
学术搜索中的文章
I Mitra, AV Nefedov, AR Brasier, RG Sadygov - Analytical chemistry, 2012